Tips for Informative A/Ci Measurements

• Relatively densely distributed points in the curvature region of an A/Ci curve are crucial for reliable parameter estimation.
• Use data from literature or exploratory measurements to establish the broad shape of A/Ci curves of similarly positioned leaves of the interested species.
• Identify the Ci range within which the curvature region is located.
• Determine the corresponding cuvette CO₂ range using either prior knowledge or preliminary measurements. As an initial try, set the cuvette CO₂ to be 1.5 - 2 times the desired Ci values.
• Sample the curvature region preferentially.