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Investigator name: First and last name
Contact information: Your email / mail addresses
Site name in full: Your site's identifier / name
Vegetation type: Mixed forest / grasslands / croplands/ etc
Soil type: Soil type at your site
Major species: List of major species at the site
Sample leaf light environment: The general light environment in which the sampled leaf is (e.g. heavily shaded from above)
Water stress assessment: Indicate whether there is water stress at the time of sampling
Instrument used: For example - Licor-6400
Extra info: Any extra information you feel would be helpful to put the sampled leaf in context
SiteID,Latitude(Degrees),Longitude(Degrees),Elevation,SampleYear,SampleDayOfYear,GrowSeasonStart,GrowSeasonEnd,StandAge,CanopyHeight,LeafAreaIndex,SpeciesSampled,AveTimeResolution,SampleHeight,LeafAge,SpecificLeafArea,LfNitrogenContent,LfCarbonContent,LfPhosphContent
NoUnit,NorthPositive,EastPositive,m,NoUnit,DayOfYear,DayOfYear,DayOfYear,Year,m,m2/m2,NoBlankSpace,Minutes,m,days,cm2/g,%,%,%
<This line contains comma-separated data described in the above two lines>
Gamma*,Kc,Ko,Alpha,Rd,gi
Pa,Pa,Pa,NoUnit,umol/m2/s,umol/m2/s/Pa
<This line contains comma-separated values (if you know) described in the above two lines. If not known fill with -9999>
Obs,HHMMSS,FTime,Photo,!AdjPhoto,!StomCond,!Ci,!Trmmol,!VpdL,Area,StmRat,BLCond,Tair,!Tleaf,TBlk,CO2R,CO2S,H2OR,H2OS,RH_R,RH_S,Flow,!PARi,PARo,Press,CsMch,HsMch,StableF,Status,PhiPS2,OxygenPress
NoUnit,HHMMSS,Second,umol/m2/s,umol/m2/s,mol/m2/s,umol/mol,mmol/m2/s,kPa,cm2,NA,mol/m2/s,oC,oC,oC,umol/mol,umol/mol,mmol/mol,mmol/mol,%,%,umol/s,umol/m2/s,umol/m2/s,Kpa,umol/mol,mmol/mol,NA,NA,NA,KPA
<This line and lines below contain comma-separated data described in the above two lines. Columns started with '!' are 'must-have' data>
1 Investigator name: First and last name
2 Contact information: Your email / mail addresses
3 Site name in full: Your site's identifier / name
4 Vegetation type: Mixed forest / grasslands / croplands/ etc
5 Soil type: Soil type at your site
6 Major species: List of major species at the site
7 Sample leaf light environment: The general light environment in which the sampled leaf is (e.g. heavily shaded from above)
8 Water stress assessment: Indicate whether there is water stress at the time of sampling
9 Instrument used: For example - Licor-6400
10 Extra info: Any extra information you feel would be helpful to put the sampled leaf in context
11 SiteID Latitude(Degrees) Longitude(Degrees) Elevation SampleYear SampleDayOfYear GrowSeasonStart GrowSeasonEnd StandAge CanopyHeight LeafAreaIndex SpeciesSampled AveTimeResolution SampleHeight LeafAge SpecificLeafArea LfNitrogenContent LfCarbonContent LfPhosphContent
12 NoUnit NorthPositive EastPositive m NoUnit DayOfYear DayOfYear DayOfYear Year m m2/m2 NoBlankSpace Minutes m days cm2/g % % %
13 <This line contains comma-separated data described in the above two lines>
14 Gamma* Kc Ko Alpha Rd gi
15 Pa Pa Pa NoUnit umol/m2/s umol/m2/s/Pa
16 <This line contains comma-separated values (if you know) described in the above two lines. If not known fill with -9999>
17 Obs HHMMSS FTime Photo !AdjPhoto !StomCond !Ci !Trmmol !VpdL Area StmRat BLCond Tair !Tleaf TBlk CO2R CO2S H2OR H2OS RH_R RH_S Flow !PARi PARo Press CsMch HsMch StableF Status PhiPS2 OxygenPress
18 NoUnit HHMMSS Second umol/m2/s umol/m2/s mol/m2/s umol/mol mmol/m2/s kPa cm2 NA mol/m2/s oC oC oC umol/mol umol/mol mmol/mol mmol/mol % % umol/s umol/m2/s umol/m2/s Kpa umol/mol mmol/mol NA NA NA KPA
19 <This line and lines below contain comma-separated data described in the above two lines. Columns started with '!' are 'must-have' data>
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Photosynthetic, Internal and Stomatal Conductance Analyses of Leaves (PISCAL)
Instruction for setting up the input file for PISCAL
-----Mentioning a brand name in this document does not constitute support for the brand-------
General instruction
- Variables with labels started with '!' must be provided in order for PISCAL to do any analysis.
For other variables, fill in with -9999 if unavailable.
- Each input file must contain only data from a single curve. Separate curves may be stored in
separate input data files and submitted at the same time.
- Save data in comma separated value format (.csv)
Line 1 to Line 10: Space provided for descriptive information about the investigator,
contact information, the site,the sample leaf and its general environmental condition
Line 11: Complementary data not used by PISCAL but needed for properly interpreting the A/Ci data and for synthesis
SiteID - (Dimensionless), Site identifier (do not leave blank between letters)
Latitude(Degrees) - Site latitude, northern hermisphere positive
Longitude(Degrees) - Site longitude, east positive
Elevation - (m), site elevation
SampleYear - (year), the year when the A/Ci data is taken
SampleDayOfYear - (day), the day of year (since 1 Jan) when the A/Ci data is taken
GrowSeasonStart - (day), the approximate start day (since 1 Jan) of growing season
GrowSeasonEnd - (day), the approximate end day (since 1 Jan) of growing season
StandAge - (year), stand age since the last disturbance
CanopyHeight - (m), the height of the canopy
LeafAreaIndex - (m2/m2), the leaf area index in the middle of growing season
SpeciesSampled - (dimensionless), the species of the leaf sample (don't leave blank between letters)
AveTimeResolution - (minutes), the average time interval between two consecutive A/Ci data points
SampleHeight - (m), the height at which the leaf is located
LeafAge - (day), the age of the leaf
SpecificLeafArea - (cm2/g), specific leaf area of the sample
LfNitrogenContent - (%), dry leaf nitrogen content of the sample
LfCarbonContent - (%), dry leaf carbon content of the sample
LfPhosphContent - (%), dry leaf phosphorus content of the sample
Line 12: The units of corresponding variables in Line 11
Line 13: The values of corresponding variables in Line 11
Line 14: The photosysnthetic model parameters you want to keep constant at the values you provide,
fill in -9999 if you want to let PISCAL decide
Gamma* - (Pa), chloroplastic CO2 photocompensation point (must be positive if provided)
Kc - (Pa), Michaelis-Menten constant for RuBP carboxylation (must be positive if provided)
Ko - (Pa), Michaelis-Menten constant for RuBP oxygenation (must be positive if provided)
Alpha - (Dimensionless), the fraction of glycolate carbon not returned to the chloroplast (must be between 0~1 if provided)
Rd - (umol/m2/s), dark respiration (must be positive if provided)
gi - (umol/m2/s/Pa), internal (also known as mesophyll) conductance
Line 15: The units of corresponding variables in Line 14
Line 16: The values of corresponding variables in Line 14
Line 17: A/Ci data for a variety of analyses, including photosynthesis, stomatal conductance,
internal conductance, water use efficiency. At this stage, we only provide to users parameters
from A/Ci curve analysis. Results from other analyses will be provided to users once they are
thoroughly tested. The format used here represents a typical output from a Licor-6400 except
for the last two columns (PhiPS2 and OxygenPress). Most variables have the same label as those
in the Licor-6400 guidebook with the same definition. Not all variables are used by PISCAL.
By using this format, however, Licor-6400 users don't have to do a lot of data organization
in order to use PISCAL. Users of other instruments could ignore columns that are not needed
for A/Ci analyses (fill in those columns with -9999) and just provide the essential variables.
Again, essential variables have labels started with '!'.
Obs: (dimensionless), the data point No.
HHMMSS - (hh:mm:ss), clock time
FTime - (seconds), number of seconds since file opened
Photo - (umol/m2/s), photosynthetic rate
!AdjPhoto - (umol/m2/s), adjusted photosynthetic rate (leakage corrected), must provide
!StomCond - (mol/m2/s), stomatal conductance, must provide
!Ci - (umol/mol), intercellular CO2 concentration (umol CO2 / mol moist air), must provide
!Trmmol - (mmol/m2/s), transpiration rate, must provide
!VpdL - (KPa), water vapor pressure deficit based on leaf temperature, must provide
Area - (cm2), leaf area
StmRat - (NA), estimate of the ratio of stomatal conductances of one side of the leaf to the other
BLCond - (mol/m2/s), total boundary layer conductance
Tair - (oC), temperature in sample cell
!Tleaf - (oC), temperature of leaf thermocouple, must provide
TBlk - (oC), IRGA Block temperature
CO2R - (umol/mol), reference CO2 concentration
CO2S - (umol/mol), Sample CO2 concentration
H2OR - (mmol/mol), reference cell water vapor concentration
H2OS - (mmol/mol), sample cell water vapor concentration
RH_R - (%), Reference cell relative humidity
RH_S - (%), sample cell relative humidity
Flow - (umol/s), molar flow rate of air entering the leaf chamber
!PARi - (umol/m2/s), PAR measured by the in-chamber quantum sensor, must provide
PARo - (umol/m2/s), PAR measured by the external quantum sensor
Press - (KPa), atmospheric pressure
CsMch - (umol/mol), sample CO2 offset
HsMch - (mmol/mol), sample H2O offset
StableF - (NA), a stability indicator as a decimal value
Status -(NA), status flag
PhiPS2 - (NA), DeltaF/Fm, the fraction of absorbed PSII photons that are used in photochemistry
OxygenPress - (Pa), atmospheric O2 partial pressure
Line 18: The units of corresponding variables in Line 17
Line 19 and below: The actual data body of the A/Ci curve
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The output file 'cntrlbestparameters.csv', which is in comma-separated-value format, contains parameters
estimated for all A/Ci curves that are submitted in the same batch. The values of these parameters are
given at the temperatures of sample leaves (that is, the estiamted parameters are not adjusted to the
same reference temperature). A curve may be fitted for multiple times, depending on what paramters are
fixed and what parameters are estimated. The first and and second partial derivatives of the cost function
with respect to the estimated paramters are also given. A valid estimation has a zero first derivative but
a non-zero second derivative.
Variables contained in the file
CurveID -(NA), the curve ID (the file name of the A/Ci curve)
ChlFlUse? -(0/1), whether or not chlorophyll fluorescence data are used for identifying the limitation states
0 = not used, 1 = used. (Currently this choice is still under evaluation and therefore ChlFlUse? = 0).
FitGi? -(0/1), whether or not the internal conductance (gi) is fitted for.
0 = not fitted and gi is either infinite or fixed at the value provided by the user.
1 = gi is estiamted
FitGamma*? - (0/1), whether or not the chloroplastic CO2 partial pressure photocompensation point is
fitted for. 0= not fitted, a prescribed value is used.
FitKco? - (0/1), whether or not the apparent Michaelis - Menten constant Kco = Kc(1+O/Ko) is fitted for.
0= not fitted, a prescribed value is calculated from Kc, Ko and the oxygen partial pressure.
1= Fitted for.
FitRd? - (0/1), whether the dark respiration is fitted for. 0= not fitted for, a prescribed value is used.
1= fitted for.
FitAlpha? - (0/1), whether alpha (the non-returned fraction of the glycolate carbon
recycled in the photorespiratory cycle) is fitted for.
0= not fitted for and alpha = 0
1= fitted for
LimitCombina - (NA), the combination of limitation states present in the A/Ci dataset. Parameters
are estimated for those limitation states that occur in the measured curve.
Rubisco: Rubisco limitation (Vcmax, Kco)
RuBP: RuBP regeneration limitation (J)
Tpu: export limitation (TPU, alpha)
MeanTleaf - (oC): average leaf temperature
MeanPARi - (umol/m2/s) average PAR inside the sample chamber.
MeanPresAir - (KPa) average atmospheric pressure
MeanPO2i - (KPa) average oxygen partial pressure
gmeso - (umol/m2/s/pa), internal CO2 transfer conductance, also called the mesophyll conductance
stargamma - (Pa), chloroplastic CO2 partial pressure photocompensation point
rdlight - (umol/m2/s), dark respiration
vcmax - (umol/m2/s), the maximal Rubisco carboxylation rate
fkco - (Pa), Kco = Kc(1+O/Ko) the apparent Michaelis-Menton constant
fjelect - (umol/m2/s), the electron transport rate at the measurement light and temperature level
tpu - (umol/m2/s), the export rate. tpu is estimated in two ways. If the dataset contains TPU limitation
points, then tpu is estimated from the regular Farquhar model fitting. If the dataset does not
contain TPU limitation points, then tpu is estimated asymptotically by fitting the A/Ci curve with
a sigmoid function: tpu= (Anet_asymptote+Rd)/3
alpha - (0 to 1), the non-returned fraction of the glycolate carbon recycled in the photorespiratory
cycle. alpha is set to 0 if not estimated.
df_gmeso - the first partial derivative of the cost function with respect to gmeso
df_stargamma - the first partial derivative of the cost function with respect to stargamma
df_rdlight- the first partial derivative of the cost function with respect to rdlight
df_vcmax- the first partial derivative of the cost function with respect to vcmax
df_fkco- the first partial derivative of the cost function with respect to fkco
df_fjelect- the first partial derivative of the cost function with respect to fjelect
df_tpu- the first partial derivative of the cost function with respect to tpu
df_alpha- the first partial derivative of the cost function with respect to alpha
df2_gmeso - the second partial derivative of the cost function with respect to gmeso
df2_stargamma - the second partial derivative of the cost function with respect to stargamma
df2_rdlight- the second partial derivative of the cost function with respect to rdlight
df2_vcmax- the second partial derivative of the cost function with respect to vcmax
df2_fkco- the second partial derivative of the cost function with respect to fkco
df2_fjelect- the second partial derivative of the cost function with respect to fjelect
df2_tpu- the second partial derivative of the cost function with respect to tpu
df2_alpha- the second partial derivative of the cost function with respect to alpha
weitedrms: (umol/m2/s), the root mean square error. For A/Ci fitting, the weighting
factor for each data point is 1 (that is, no weighting)
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The file 'cntrlcomparison.csv', which is in comma-separated-value format, contains outputs from PISCAL that
facilitates examination of how well the fitting is. First model prediction is calculated at each sampling
point. Then model prediction is calculated at many selected levels of intercelluar CO2 partial pressure
levels under three limitation states to enable plotting curves. The same structure is then repeated for
each fitting of the same curve with different parameters to be estimated and with different curves. Note
that some common information about a curve is repeated for each data point to make the structure of the
file as regular as possible.
!The first section compares model calculation at the optimized parameters with measurements
CurveID - (NA), the curve identifier, repeated for each point
ChlFlUse? -(0/1), whether or not chlorophyll fluorescence data are used for identifying the limitation states
0 = not used, 1 = used. (Currently this choice is still under evaluation and therefore ChlFlUse? = 0).
FitGi? _(0/1), whether or not the internal conductance (gi) is fitted for.
0 = not fitted and gi is either infinite or fixed at the value provided by the user.
1 = gi is estiamted, repeated for each point
FitGamma*? - (0/1), whether or not the chloroplastic CO2 partial pressure photocompensation point is
fitted for. 0= not fitted, a prescribed value is used, repeated for each point
FitKco? - (0/1), whether or not the apparent Michaelis - Menten constant Kco = Kc(1+O/Ko) is fitted for.
0= not fitted, a prescribed value is calculated from Kc, Ko and the oxygen partial pressure.
1= Fitted for, repeated for each point
FitRd? - (0/1), whether the dark respiration is fitted for. 0= not fitted for, a prescribed value is used.
1= fitted for. repeated for each point
FitAlpha? - (0/1), whether alpha (the non-returned fraction of the glycolate carbon
recycled in the photorespiratory cycle) is fitted for.
0= not fitted for and alpha = 0
1= fitted for, repeated for each point
LimitCombina - (NA), the combination of limitation states present in the A/Ci dataset. Parameters
are estimated for those limitation states that occur in the measured curve, repeated for each point
Rubisco: Rubisco limitation (Vcmax, Kco)
RuBP: RuBP regeneration limitation (J)
Tpu: export limitation (TPU, alpha)
PCO2i - (Pa), intercellular CO2 partial pressure
PCO2c - (Pa), chloroplastic CO2 partial pressure corresponding to the PCO2i of a point.
PCO2c=PCO2i-AnetCal/internal conductance
AnetMeas - (umol/m2/s), measured net assimilation rate
AnetCal - (umol/m2/s), calculated net assimilation rate
weitedrms - (umol/m2/s), root mean square error of the fitting (weiting coefficient =1)
PointLimitType - (1,2,3). 1 = point limited by rubisco
2 = point limited by RuBP regeneration
3 = point limited by TPU
!The second section gives photosynthesis for each of the three limitation states at selected values of intercellular
!partial pressure. Note that the corresponding values of chloraplastic CO2 partial pressure depend on the limitation state.
CO2i - (Pa), intercellular CO2 partial pressure
CO2cc- (Pa), Chloraplastic CO2 partial pressure for Rubisco limited photosynthesis
Ac- (umol/m2/s), Rubisco-limited net assimilation rate calculated with the optimized parameters
CO2cj- (Pa), Chloraplastic CO2 partial pressure for RuBP regeneration limited photosynthesis
Aj- (umol/m2/s), RuBP regeneration-limited net assimilation rate calculated with estimated parameters
CO2ct- (Pa), Chloraplastic CO2 partial pressure for export limited photosynthesis
At- (umol/m2/s), TPU-limited net assimilation rate calculated with estimated parameters
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The file 'cntrlparameters.csv' is an extended version of the output file 'cntrlbestparameters.csv'
Variables contained in the file:
CurveID -(NA), the curve ID (the file name of the A/Ci curve)
ChlFlUse? -(0/1), whether or not chlorophyll fluorescence data are used for identifying the limitation states
0 = not used, 1 = used. (Currently this choice is still under evaluation and therefore ChlFlUse? = 0).
FitGi? _(0/1), whether or not the internal conductance (gi) is fitted for.
0 = not fitted and gi is either infinite or fixed at the value provided by the user.
1 = gi is estiamted
FitGamma*? - (0/1), whether or not the chloroplastic CO2 partial pressure photocompensation point is
fitted for. 0= not fitted, a prescribed value is used.
FitKco? - (0/1), whether or not the apparent Michaelis - Menten constant Kco = Kc(1+O/Ko) is fitted for.
0= not fitted, a prescribed value is calculated from Kc, Ko and the oxygen partial pressure.
1= Fitted for.
FitRd? - (0/1), whether the dark respiration is fitted for. 0= not fitted for, a prescribed value is used.
1= fitted for.
FitAlpha? - (0/1), whether alpha (the non-returned fraction of the glycolate carbon
recycled in the photorespiratory cycle) is fitted for.
0= not fitted for and alpha = 0
1= fitted for
LimitCombina - (NA), the combination of limitation states present in the A/Ci dataset. Parameters
are estimated for those limitation states that occur in the measured curve.
Rubisco: Rubisco limitation (Vcmax, Kco)
RuBP: RuBP regeneration limitation (J)
Tpu: export limitation (TPU, alpha)
Vcmax25 - (umol/m2/s), the maximal Rubisco carboxylation rate at 25 oC. It is related to vcmax at the
leaf temperature TLeaf (K) through the following expression:
vcmax=Vcmax25*exp(f3)*(1+exp(f2))/(1+exp(f1))
where f1=(sv_vcmax*TLeaf-hd_vcmax)/(R*TLeaf)
f2=(sv_vcmax*TRef-hd_vcmax)/(R*TRef)
f3=ha_vcmax*(1-TRef/TLeaf)/(R*TRef)
TRef is the reference temperature at 25+273.15 K, R is uiversal gas constant in kJmol-1K-1
ha_vcmax: The activation energy (Ha) in Vcmax temperature response function [kJmol-1]
hd_vcmax: The deactivation energy (Hd) in Vcmax temperature response function [kJmol-1]
sv_vcmax: The entropy term (Sv) in Vcmax temperature response function [kJmol-1K-1]
Jmax25 - (umolm-2s-1), the maximal electron transport rate at the 25 oC. Jmax25 is calculated
from the electron transport rate at a given level of light and leaf temperature based on
a series of relationships from Bernacchi C.J., Pimentel C. & Long S.P.(2003) In vivo temperature
response functions of parameters required to model RuBP-limited photosynthesis. Plant Cell
and Environment 26, 1419-1430.
ha_jmax: The activation energy (Ha) in jmax temperature response function [kJmol-1]
hd_jmax: The deactivation energy (Hd) in jmax temperature response function [kJmol-1]
sv_jmax: The entropy term (Sv) in jmax temperature response function [kJmol-1K-1]
Rdlight25 - (umolm-2s-1), the dark respiration rate at 25 oC. It is related to Rdlight through
the following relationship:
Rdlight=Rdlight25*exp((ha_darkresp/R)*(1/298.15-1/templeaf))
R is uiversal gas constant in kJmol-1K-1
ha_darkresp: parameter in the temperature response function of dark respiration [kJmol-1]
gmeso25: (umolm-2s-1Pa-1). CO2 internal (mesophyll) transfer conductance at 25 oC. It is related
to gmeso through the following relationship:
gmeso=gmeso25*exp(f3)*(1+exp(f2))/(1+exp(f1))
where f1=(sv_gmeso*TLeaf-hd_gmeso)/(R*TLeaf)
f2=(sv_gmeso*TRef-hd_gmeso)/(R*TRef)
f3=ha_gmeso*(1-TRef/TLeaf)/(R*TRef)
ha_gmeso: The activation energy (Ha) in gmeso temperature response function [kJmol-1]
hd_gmeso: The deactivation energy (Hd) in gmeso temperature response function [kJmol-1]
sv_gmeso: The entropy term (Sv) in gmeso temperature response function [kJmol-1K-1]
tpu25 - (umolm-2s-1), the Triose Phosphate Utilization (TPU) rate at 25 oC. If there are no
tpu limitation points in the A/Ci dataset, it is calculated from the asymptotically estimated
tpu.
tpu=tpu25*exp(f3)*(1+exp(f2))/(1+exp(f1))
where f1=(sv_tpu*TLeaf-hd_tpu)/(R*TLeaf)
f2=(sv_tpu*TRef-hd_tpu)/(R*TRef)
f3=ha_tpu*(1-TRef/TLeaf)/(R*TRef)
ha_tpu: The activation energy (Ha) in tpu temperature response function [kJmol-1]
hd_tpu: The deactivation energy (Hd) in tpu temperature response function [kJmol-1]
sv_tpu: The entropy term (Sv) in tpu temperature response function [kJmol-1K-1]
gammas*25: (Pa). Chloroplastic CO2 photocompensation point at the reference temperature 25 oC.
stargamma=gammas25*dexp((ha_stargamma/R)*(1/298.15-1/TLeaf))
ha_stargamma: parameter in the temperature response function of the co2 compensation point [kJmol-1]
fkc25: (Pa), Michaelis - Menten constant for CO2 at 25 oC
fkc=fkc25*dexp((ha_kc/R)*(1/298.15-1/TLeaf))
fko25: (Pa), Michaelis - Menten constant for O2 at 25 oC
fko=fko25*dexp((ha_ko/R)*(1/298.15-1/TLeaf))
ha_kc: a CO2 Michaelis temp coefficient [kJmol-1]
ha_ko: a O2 Michaelis temp coefficient [kJmol-1]
alpha: (0-1), the fraction of glycolate carbon not returned to the chloroplat [0-1]
numrubisco1cur - (NA), total number of points limited by Rubisco
numrubp1curve - (NA), total number of points limited by RuBP regeneration
numtpu1curve - (NA), total number of points limited by TPU
weitedrms1curve - (umolm-2s-1), the root mean square for the fitting
gamma25: (Pa), the intercellular CO2 partial pressure at which Anet = 0 for leaf temperature at
25 oC. Note that at this point, intercellular CO2 partial pressure = Chloroplastic CO2
fkco25: (Pa), fkco25 = fkc25*(1+O/fko25)
leaftemp - (oC): average leaf temperature
MeanPARi - (umol/m2/s) average PAR inside the sample chamber.
MeanPresAir - (KPa) average atmospheric pressure
MeanPO2i - (KPa) average oxygen partial pressure
gmeso - (umol/m2/s/pa), internal CO2 transfer conductance, also called the mesophyll conductance
Gamma*: (Pa), the chloroplastic CO2 compensation point.
CalcedGamma: (Pa), the intercellular CO2 partial pressure at which Anet = 0 for a given leaf
temperature. Note that at this point, intercellular CO2 partial pressure = Chloroplastic CO2.
Obtained from the fitted Farquhar model.
vcmax - (umol/m2/s), the maximal Rubisco carboxylation rate at the measurement leaf temperature
fkco - (Pa), Kco = Kc(1+O/Ko) the apparent Michaelis-Menton constant, either estimated from the
the A/Ci data or calculated from prescribed Kc and Ko. If it is not estimated from
the A/Ci data, then fkco = fkco_mod
fkco_mod - (Pa), Kco = Kc(1+O/Ko) the apparent Michaelis-Menton constant, calculated
from prescribed Kc and Ko.
fjelect: (umolm-2s-1), the electron transport rate at the measurement leaf temperature
fjmax: (umolm-2s-1), the maximal electron transport rate the measurement leaf temperature
tpu: (umolm-2s-1), the triose phosphaste utilization rate at the measurement leaf temperature. When
there are no tpu points in the A/Ci dataset, it is calculated aymptotically.
stargamma25_ori: (Pa), the prescribed chloroplastic CO2 photocompensation point at 25 oC.
Rdlight: (umolm-2s-1), the dark respiration rate at the measurement leaf temperature.
CO2i1Thres25: (Pa), the intercellular CO2 value at the transition between Rubisco limitation and
the RuBP regeneration- limitation transition at 25 oC.
Anet25_CO2i1: (umolm-2s-1), the Anet at CO2i1Thres25
CO2i2Thres25: (Pa), the intercellular CO2 value at the transition between RuBP regeneration- and
TPU- limitation transition at 25 oC
Anet25_CO2i2: (umolm-2s-1), Anet at CO2i2Thres25
CO2i1Thres: (Pa), the intercellular CO2 value at the transition between Rubisco limitation and
the RuBP regeneration- limitation transition at the measurement leaf temperature.
Anet_CO2i1: (umolm-2s-1), Anet at CO2i1Thres
CO2i2Thres: (Pa), the intercellular CO2 value at the transition between RuBP regeneration- and
TPU- limitation transition at the measurement leaf temperature.
Anet_CO2i2: (umolm-2s-1), Anet at CO2i2Thres
AsmptoticAnet: (umolm-2s-1), the asymptote of the A/Ci curve
df_gmeso - the first partial derivative of the cost function with respect to gmeso
df_stargamma - the first partial derivative of the cost function with respect to stargamma
df_rdlight- the first partial derivative of the cost function with respect to rdlight
df_vcmax- the first partial derivative of the cost function with respect to vcmax
df_fkco- the first partial derivative of the cost function with respect to fkco
df_fjelect- the first partial derivative of the cost function with respect to fjelect
df_tpu- the first partial derivative of the cost function with respect to tpu
df_alpha- the first partial derivative of the cost function with respect to alpha
df2_gmeso - the second partial derivative of the cost function with respect to gmeso
df2_stargamma - the second partial derivative of the cost function with respect to stargamma
df2_rdlight- the second partial derivative of the cost function with respect to rdlight
df2_vcmax- the second partial derivative of the cost function with respect to vcmax
df2_fkco- the second partial derivative of the cost function with respect to fkco
df2_fjelect- the second partial derivative of the cost function with respect to fjelect
df2_tpu- the second partial derivative of the cost function with respect to tpu
df2_alpha- the second partial derivative of the cost function with respect to alpha
!--------------------------------------------------------------------------
The following variables are copied from the A/Ci data files users provide
SiteID - (Dimensionless), Site identifier (do not leave blank between letters)
Latitude(Degrees) - Site latitude, northern hermisphere positive
Longitude(Degrees) - Site longitude, east positive
Elevation - (m), site elevation
SampleYear - (year), the year when the A/Ci data is taken
SampleDayOfYear - (day), the day of year (since 1 Jan) when the A/Ci data is taken
GrowSeasonStart - (day), the approximate start day (since 1 Jan) of growing season
GrowSeasonEnd - (day), the approximate end day (since 1 Jan) of growing season
StandAge - (year), stand age since the last disturbance
CanopyHeight - (m), the height of the canopy
LeafAreaIndex - (m2/m2), the leaf area index in the middle of growing season
SpeciesSampled - (dimensionless), the species of the leaf sample (don't leave blank between letters)
AveTimeResolution - (minutes), the average time interval between two consecutive A/Ci data points
SampleHeight - (m), the height at which the leaf is located
LeafAge - (day), the age of the leaf
SpecificLeafArea - (cm2/g), specific leaf area of the sample
LfNitrogenContent - (%), dry leaf nitrogen content of the sample
LfCarbonContent - (%), dry leaf carbon content of the sample
LfPhosphContent - (%), dry leaf phosphorus content of the sample
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Investigator name: First and last name
Contact information: Your email / mail addresses
Site name in full: Your site's identifier / name
Vegetation type: Mixed forest / grasslands / croplands/ etc
Soil type: Soil type at your site
Major species: List of major species at the site
Sample leaf light environment: The general light environment in which the leaf is in (e.g. heavily shaded from above)
Water stress assessment: Indicate whether there is water stress at the time of sampling
Instrument used: For example - Licor-6400
Extra info: Any extra information you feel would be helpful to put the sampled leaf in context
SiteID,Latitude(Degrees),Longitude(Degrees),Elevation,SampleYear,SampleDayOfYear,GrowSeasonStart,GrowSeasonEnd,StandAge,CanopyHeight,LeafAreaIndex,SpeciesSampled,AveTimeResolution,SampleHeight,LeafAge,SpecificLeafArea,LfNitrogenContent,LfCarbonContent,LfPhosphContent
NoUnit,NorthPositive,EastPositive,m,NoUnit,DayOfYear,DayOfYear,DayOfYear,Year,m,m2/m2,NoBlankSpace,Minutes,m,days,cm2/g,%,%,%
MOFLUX,38.733,-92.2,237.4,2008,174,105,270,70,17,4.2,WhiteOak,3,0.9144,69,203.72,-9999,-9999,-9999
Gamma*,Kc,Ko,Alpha,Rd,gi
Pa,Pa,Pa,NoUnit,umol/m2/s,umol/m2/s/Pa
-9999,-9999,-9999,-9999,-9999,-9999
Obs,HHMMSS,FTime,Photo,!AdjPhoto,!StomCond,!Ci,!Trmmol,!VpdL,Area,StmRat,BLCond,Tair,!Tleaf,TBlk,CO2R,CO2S,H2OR,H2OS,RH_R,RH_S,Flow,!PARi,PARo,Press,CsMch,HsMch,StableF,Status,PhiPS2,OxygenPress
NoUnit,HHMMSS,Second,umol/m2/s,umol/m2/s,mol/m2/s,umol/mol,mmol/m2/s,kPa,cm2,NA,mol/m2/s,oC,oC,oC,umol/mol,umol/mol,mmol/mol,mmol/mol,%,%,umol/s,umol/m2/s,umol/m2/s,Kpa,umol/mol,mmol/mol,NA,NA,NA,KPA
13,14:44:20,664.5,7.51,7.470041223,0.149,279,2.64,1.74,2,1,9.29,25.02,26.93,23.62,374.86,370.62,17.176,18.471,53.32,57.34,400.7,1201,47,98.83,2.63,0.43,0.2,111115,0.093,-9999
19,14:46:49,813.5,5.87,5.855008277,0.155,230,2.74,1.73,2,1,9.29,25.33,26.95,24.22,302.85,299.51,17.244,18.589,52.55,56.64,400.7,1201,48,98.82,2.51,0.416,0.8,111115,0.088,-9999
25,14:49:19,962.5,4.98,4.986692181,0.158,192,2.78,1.72,2,1,9.29,25.25,26.95,24.02,251.75,248.92,17.346,18.709,53.13,57.3,400.7,1201,43,98.82,2.54,0.408,0.5,111115,0.081,-9999
31,14:51:48,1111.5,3.58,3.613166391,0.167,159,2.9,1.7,2,1,9.29,25.22,26.91,24.02,200.66,198.59,17.405,18.823,53.4,57.75,400.7,1200,41,98.82,2.46,0.403,0.5,111115,0.075,-9999
37,14:54:17,1260.5,2.27,2.337349887,0.175,123,3.06,1.71,2,1,9.29,25.26,26.99,24.03,149.71,148.35,17.397,18.895,53.22,57.81,400.7,1199,43,98.82,2.34,0.393,0.5,111115,0.069,-9999
43,14:56:46,1410.5,0.887,1.001965238,0.184,88.3,3.2,1.7,2,1,9.29,25.35,26.98,24.22,99.42,98.82,17.373,18.939,52.87,57.64,400.7,1199,44,98.82,2.28,0.383,1,111115,0.061,-9999
49,14:59:15,1559.5,-0.542,-0.343159241,0.189,51.6,3.28,1.7,2,1,9.29,25.37,26.98,24.21,48.11,48.31,17.366,18.973,52.79,57.67,400.7,1199,45,98.82,2.14,0.376,0.8,111115,0.052,-9999
61,15:07:44,2068.5,7.01,6.969621394,0.134,278,2.41,1.76,2,1,9.29,25.03,27.03,23.63,377.22,371.95,16.919,18.495,52.47,57.36,300.1,1199,49,98.82,2.65,0.438,0.2,111115,0.09,-9999
68,15:11:13,2277.5,8.46,8.390629343,0.116,347,2.14,1.78,2,1,9.29,24.93,27.05,23.43,482.66,476.34,16.879,18.276,52.67,57.03,300.1,1198,47,98.81,2.74,0.458,0.8,111115,0.098,-9999
75,15:14:42,2486.5,8.98,8.883476363,0.0729,387,1.38,1.83,2,1,9.29,24.64,27.04,23.04,607.06,600.53,16.913,17.813,53.72,56.57,300.1,1198,46,98.81,2.71,0.479,0.8,111115,0.102,-9999
82,15:18:10,2694.5,9.64,9.509884017,0.0453,439,0.868,1.85,2,1,9.29,24.48,27.04,22.85,806.75,799.86,16.997,17.565,54.49,56.31,300.1,1198,99,98.81,2.37,0.491,0.5,111115,0.102,-9999
89,15:21:36,2900.5,10.4,10.24385533,0.0318,454,0.609,1.85,2,1,9.29,24.13,26.92,22.16,1006.08,998.77,16.958,17.357,55.52,56.83,300.1,1198,64,98.8,1.78,0.512,0.5,111115,0.1,-9999
96,15:25:02,3106.5,10.3,10.12230686,0.0259,533,0.501,1.86,2,1,9.29,24.11,26.98,22.26,1207.66,1200.37,16.973,17.301,55.61,56.69,300.1,1202,50,98.8,1.46,0.531,0.8,111115,0.101,-9999
1 Investigator name: First and last name
2 Contact information: Your email / mail addresses
3 Site name in full: Your site's identifier / name
4 Vegetation type: Mixed forest / grasslands / croplands/ etc
5 Soil type: Soil type at your site
6 Major species: List of major species at the site
7 Sample leaf light environment: The general light environment in which the leaf is in (e.g. heavily shaded from above)
8 Water stress assessment: Indicate whether there is water stress at the time of sampling
9 Instrument used: For example - Licor-6400
10 Extra info: Any extra information you feel would be helpful to put the sampled leaf in context
11 SiteID Latitude(Degrees) Longitude(Degrees) Elevation SampleYear SampleDayOfYear GrowSeasonStart GrowSeasonEnd StandAge CanopyHeight LeafAreaIndex SpeciesSampled AveTimeResolution SampleHeight LeafAge SpecificLeafArea LfNitrogenContent LfCarbonContent LfPhosphContent
12 NoUnit NorthPositive EastPositive m NoUnit DayOfYear DayOfYear DayOfYear Year m m2/m2 NoBlankSpace Minutes m days cm2/g % % %
13 MOFLUX 38.733 -92.2 237.4 2008 174 105 270 70 17 4.2 WhiteOak 3 0.9144 69 203.72 -9999 -9999 -9999
14 Gamma* Kc Ko Alpha Rd gi
15 Pa Pa Pa NoUnit umol/m2/s umol/m2/s/Pa
16 -9999 -9999 -9999 -9999 -9999 -9999
17 Obs HHMMSS FTime Photo !AdjPhoto !StomCond !Ci !Trmmol !VpdL Area StmRat BLCond Tair !Tleaf TBlk CO2R CO2S H2OR H2OS RH_R RH_S Flow !PARi PARo Press CsMch HsMch StableF Status PhiPS2 OxygenPress
18 NoUnit HHMMSS Second umol/m2/s umol/m2/s mol/m2/s umol/mol mmol/m2/s kPa cm2 NA mol/m2/s oC oC oC umol/mol umol/mol mmol/mol mmol/mol % % umol/s umol/m2/s umol/m2/s Kpa umol/mol mmol/mol NA NA NA KPA
19 13 14:44:20 664.5 7.51 7.470041223 0.149 279 2.64 1.74 2 1 9.29 25.02 26.93 23.62 374.86 370.62 17.176 18.471 53.32 57.34 400.7 1201 47 98.83 2.63 0.43 0.2 111115 0.093 -9999
20 19 14:46:49 813.5 5.87 5.855008277 0.155 230 2.74 1.73 2 1 9.29 25.33 26.95 24.22 302.85 299.51 17.244 18.589 52.55 56.64 400.7 1201 48 98.82 2.51 0.416 0.8 111115 0.088 -9999
21 25 14:49:19 962.5 4.98 4.986692181 0.158 192 2.78 1.72 2 1 9.29 25.25 26.95 24.02 251.75 248.92 17.346 18.709 53.13 57.3 400.7 1201 43 98.82 2.54 0.408 0.5 111115 0.081 -9999
22 31 14:51:48 1111.5 3.58 3.613166391 0.167 159 2.9 1.7 2 1 9.29 25.22 26.91 24.02 200.66 198.59 17.405 18.823 53.4 57.75 400.7 1200 41 98.82 2.46 0.403 0.5 111115 0.075 -9999
23 37 14:54:17 1260.5 2.27 2.337349887 0.175 123 3.06 1.71 2 1 9.29 25.26 26.99 24.03 149.71 148.35 17.397 18.895 53.22 57.81 400.7 1199 43 98.82 2.34 0.393 0.5 111115 0.069 -9999
24 43 14:56:46 1410.5 0.887 1.001965238 0.184 88.3 3.2 1.7 2 1 9.29 25.35 26.98 24.22 99.42 98.82 17.373 18.939 52.87 57.64 400.7 1199 44 98.82 2.28 0.383 1 111115 0.061 -9999
25 49 14:59:15 1559.5 -0.542 -0.343159241 0.189 51.6 3.28 1.7 2 1 9.29 25.37 26.98 24.21 48.11 48.31 17.366 18.973 52.79 57.67 400.7 1199 45 98.82 2.14 0.376 0.8 111115 0.052 -9999
26 61 15:07:44 2068.5 7.01 6.969621394 0.134 278 2.41 1.76 2 1 9.29 25.03 27.03 23.63 377.22 371.95 16.919 18.495 52.47 57.36 300.1 1199 49 98.82 2.65 0.438 0.2 111115 0.09 -9999
27 68 15:11:13 2277.5 8.46 8.390629343 0.116 347 2.14 1.78 2 1 9.29 24.93 27.05 23.43 482.66 476.34 16.879 18.276 52.67 57.03 300.1 1198 47 98.81 2.74 0.458 0.8 111115 0.098 -9999
28 75 15:14:42 2486.5 8.98 8.883476363 0.0729 387 1.38 1.83 2 1 9.29 24.64 27.04 23.04 607.06 600.53 16.913 17.813 53.72 56.57 300.1 1198 46 98.81 2.71 0.479 0.8 111115 0.102 -9999
29 82 15:18:10 2694.5 9.64 9.509884017 0.0453 439 0.868 1.85 2 1 9.29 24.48 27.04 22.85 806.75 799.86 16.997 17.565 54.49 56.31 300.1 1198 99 98.81 2.37 0.491 0.5 111115 0.102 -9999
30 89 15:21:36 2900.5 10.4 10.24385533 0.0318 454 0.609 1.85 2 1 9.29 24.13 26.92 22.16 1006.08 998.77 16.958 17.357 55.52 56.83 300.1 1198 64 98.8 1.78 0.512 0.5 111115 0.1 -9999
31 96 15:25:02 3106.5 10.3 10.12230686 0.0259 533 0.501 1.86 2 1 9.29 24.11 26.98 22.26 1207.66 1200.37 16.973 17.301 55.61 56.69 300.1 1202 50 98.8 1.46 0.531 0.8 111115 0.101 -9999
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