# Tips for Informative A/Ci Measurements

- Relatively densely distributed points in the curvature region of an A/Ci curve are crucial for reliable parameter estimation.
- Use data from literature or exploratory measurements to establish the broad shape of A/Ci curves of similarly positioned leaves of the interested species.
- Identify the Ci range within which the curvature region is located.
- Determine the corresponding cuvette CO2 range using either prior knowledge or preliminary measurements. As an initial try, set the cuvette CO2 to be 1.5 - 2 times the desired Ci values.
- Sample the curvature region preferentially.